Online coupling of reverse-phase and hydrophilic interaction liquid chromatography for protein and glycoprotein characterization
نویسندگان
چکیده
منابع مشابه
Online coupling of reverse-phase and hydrophilic interaction liquid chromatography for protein and glycoprotein characterization
We have developed a novel system for coupling reverse-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) online in a micro-flow scheme. In this approach, the inherent solvent incompatibility between RP and HILIC is overcome through the use of constant-pressure online solvent mixing, which allows our system to perform efficient separations of both hydrophilic and hydrophobic co...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2010
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-010-3991-2